Genotyping and investigation of antibiotic resistance genes of Acinetobacter baumannii isolates isolated from raw meat using RAPD-PCR method

Nadereh Askari Khashouia; Fatemeh  Momtaz; Mahdieh  Momtaz; Seyed Mehdi Ghasemi

doi:10.61882/jcbior.6.4.329

Authors

Nadereh Askari Khashouia Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran
Fatemeh Momtaz Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Mahdieh Momtaz Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Seyed Mehdi Ghasemi Department of Biotechnology, Faculty of Biological Sciences and Technology, Shahid Ashrafi Esfahani University, Isfahan, Iran

DOI:

https://doi.org/10.61882/jcbior.6.4.329

Keywords:

Genotyping, Antibiotic resistance, Acinetobacter baumannii, Carbapenemase, , RAPD-PCR

Abstract

Acinetobacter baumannii is a well-known pathogen linked to hospital infections and has recently been detected in raw meat from livestock and poultry, raising concerns over antibiotic resistance transmission to humans. Considering the extensive prevalence of multidrug-resistant A. baumannii in food sources, this study focused on genotyping A. baumannii isolates from meat and poultry samples and examining the presence of essential antibiotic resistance genes. As part of a cross-sectional study conducted in August 2023, researchers gathered 125 raw meat samples from livestock and poultry at various sites throughout Isfahan province, Iran. The A. baumannii isolates were identified by standard microbiological producers and examined for antimicrobial susceptibility using the disk diffusion technique. Their resistance patterns were analyzed through RAPD-PCR. Totally, 22 A. baumannii isolates from raw meat were recovered. All isolates exhibited resistance to at least two antibiotics. Strains derived from livestock showed high resistance to tetracycline (72.7%), whereas strains from poultry exhibited the highest resistance to both tetracycline (90.9%), and gentamicin (81.8%). In both poultry and livestock isolates, tetA was the most frequently detected resistance gene at 90.1 and 81.8%, respectively. While, the bla_oxa-24-like genes were present at one isolate with a rate of 9.1%. Genotyping grouped the isolates into five RAPD profiles, with genetic similarities ranging from 74%-100%, indicating notable diversity despite widespread resistance. These findings highlight the emergence of antibiotic-resistant A. baumannii in meat products and underscore the potential risk of resistance gene transfer from animals to humans, emphasizing the need for further research and public health strategies.

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